Abstract:[Objective] This study aimed to establish the cryopreservation technique of Spirulina that with low temperature tolerance and research the preservative applicability of this method to the different Spirulina. [Methods] We screened out the low temperature tolerant Spirulina by iodometric method. This paper used the single factor analysis and orthogonal experimental designed method to optimize the conditions to the cryopreservation technique of Spirulina with low temperature tolerance, and then used this optimized method to preserve the eight different kinds of Spirulina. [Results] The result showed that the FACHB-351 Spirulina was the best one to endure the low temperature, and the optimal cryopreservation conditions were obtained as follows: using the 10% sucrose solution as the cryoprotectant, putting the algal cell density of 1.0×107 CFU/mL in the 4 °C refrigerator liquid and cultivating for 72 h, mixing up the algae fluid and protective agent after precooling at 0 °C for 30 minutes respectively, and then preserving it in the liquid nitrogen after the mixture liquid stayed at 0 °C for 3 h. This experimental result indicated that FACHB-350, FACHB-1070, FACHB-902 were no-resistant to low temperature and lost cell activity with the survival rate of 0% after preservation for six months, the rest five kinds of Spirulina could resurrect during a certain period of time for the growth of normal. And the FACHB-351 showed be the highest survival rate 39.33%. [Conclusion] This study established the cryopreservation method which was suitable for the preservation of good low temperature resistance Spirulina.
