Abstract:Based on the principle that content is the core factor of a course, the problems in both teaching and teaching content reforms were analyzed in developing food microbe detecting abilities of the students in food nutrition and detection major in higher vocational colleges. According to the actual requirements and occupation standards of food industry, the necessity and urgency of the teaching content reform were expounded and what contents should be included in teaching was elaborated. Great importance was attached to the construction of teaching contents regarding food microbe detection in order to enhance students’ abilities to detect microbes in food. In teaching practice, the comprehensively constructed teaching contents could attain above training objective.