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氨氮和硝酸盐氮对沙雷氏菌S2还原Cr(Ⅵ)能力的影响
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重庆市自然科学基金计划项目(No. 2010BB5360)


Effects of ammonia and nitrate nitrogen on Cr(Ⅵ) reduction ability of Serratia sp. S2
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    摘要:

    【目的】研究氨氮(AN)与硝酸盐氮(NN)对沙雷氏菌S2还原Cr(Ⅵ)能力的影响。【方法】在实验室中模拟常见的环境中氮污染,S2在含Cr(Ⅵ)培养的同时在培养体系中加入不同剂量的AN或/和NN,每隔一定时间测定培养体系的菌量(A600)、Cr(Ⅵ)还原率、AN含量、NN含量。【结果】低、中浓度AN能缓解Cr(Ⅵ)对S2生长的抑制作用;高浓度NN和AN可加快S2的衰亡。AN独立作用时,各组间Cr(Ⅵ)去除率和氨氮含量无显著关联。NN独立作用时,S2的Cr(Ⅵ)去除率在低浓度组降低10.0%以上(P<0.05),在高浓度组增高7.1% (P<0.05);S2能在4 h内使200 mg/L的NN降至对照组水平。双氮联合作用时,低浓度组对菌株除Cr(Ⅵ)能力的影响与AN单独作用类似,而高浓度组则类似NN单独作用。【结论】AN的存在对S2的Cr(Ⅵ)还原能力无明显影响,NN浓度高低对S2的Cr(Ⅵ)还原能力有不同影响,S2具有很强的除NN能力,可同时去除环境中Cr(Ⅵ)和硝酸盐氮污染。

    Abstract:

    [Objective] To study the effect of Cr(Ⅵ) reduction ability of Serratia sp. S2 under ammonia nitrogen (AN) and nitrate nitrogen (NN) with different concentration. [Methods] Simulated the nitrogen pollution of common environment in the laboratory and added different doses of AN or/and NN to the cultivation system in order to evaluate the effect of different types and concentrations of nitrogen, S2 was cultured in the premise of the Cr(Ⅵ) containing culture. Shake culture at constant 37 °C and measure A600, removal rate of Cr(Ⅵ), amount of AN and NN at a regular intervals. [Results] The growth inhibition of Cr(Ⅵ) to S2 was remittenced under the low and middle AN groups. The decline of S2 was accelerated under the high AN and NN groups. The removal rate of Cr(Ⅵ) and amount of AN among experience and control groups according to the independent effect of AN had no significant relationship. The removal rate of Cr(Ⅵ) of low concentration groups had a significant reduction of more than 10.0% and a increase of 7.1% in high concentration groups according to the independent effect of NN. S2 had the ability to reduce 200 mg/L NN to the level of control groups within 4 h. While AN and NN effect at the same time, AN played a leading role in low concentration groups and NN in high groups during the course of Cr(Ⅵ) reduction. [Conclusion] The presence of AN had no significant effect to the Cr(Ⅵ)-removal ability. Different concentrations of NN had different effect to Cr(Ⅵ)-removal ability. High concentration groups had an advantage effect. S2 had a significant ability of reducing NN.

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吴颖,邓鹏,贾燕,白群华,肖虹. 氨氮和硝酸盐氮对沙雷氏菌S2还原Cr(Ⅵ)能力的影响[J]. 微生物学通报, 2016, 43(2): 270-278

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  • 在线发布日期: 2016-01-26
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