人工合成启动子文库研究进展

Progress in synthetic promoter library

摘要:

随着合成生物学的发展，人工合成功能元件在代谢工程领域显示出巨大应用潜力。启动子是调控基因转录水平的重要元件，可以利用人工合成启动子文库对代谢途径进行精细调控，使基因适量表达，实现代谢途径的组合优化。文章综述了人工合成启动子文库的研究进展，评述了不同文库的构建策略，讨论了人工合成启动子文库在代谢工程领域的应用，并展望了人工合成启动子文库的发展方向。

Abstract:

With the development of synthetic biology, design and synthesis of functional elements show great potential in assisting and simplifying the study of metabolic engineering. The fundamental level of transcriptional control takes place at promoter elements that drive gene expression. Synthetic promoter library (SPL) provides promoter with different strength for fine-tuned gene expression. SPL is an important solution to optimal expression of gene and combinatorial optimization of metabolic pathways. This review is mainly focused on the current methods and advances in strategies of SPL. Apart from the description of the developments, we discuss the application of artificial promoter in metabolic engineering and research prospect of promoter engineering in the future.