基于高通量测序技术研究水貂远端肠道细菌群落组成(英文)

Determination of bacterial community composition in the distal gut of mink (Mustela vison) by high-throughput sequencing

摘要:

【目的】研究水貂远端肠道微生物群落组成及其多样性。【方法】通过高通量测序技术研究水貂远端肠内容物细菌的组成和多样性。【结果】从10只健康水貂远端肠道内容物样品中得到146 287高质量序列代表17个菌门、167个细菌属。水貂肠道细菌以厚壁菌门(59.99%)、拟杆菌门(16.2%)、梭杆菌门(11.5%)、放线菌门(5.9%)和变形菌门(5.3%)为主，其中厚壁菌门最为丰富。厚壁菌门中的梭菌目是最丰富的目，而梭菌目中的链球菌占有50%以上的OTUs，是最大的细菌属。【结论】水貂肠道内存在复杂的微生物区系，这对进一步研究水貂对营养物质吸收利用提供了理论基础。

Abstract:

[Objective] The aim of the study was to determine the composition and diversity of microbial communities in the distal gut of mink (Mustela vison). [Methods] The composition and diversity of distal gut content bacteria were investigated by the high-throughput sequencing. [Results] A total of 146 287 filtered high-quality representing 17 phylum and 167 genus bacterial phylotypes (operational taxonomical units, OTUs) were identified in distal gut contents samples from 10 healthy mink. There are mainly Firmicutes (59.99%), Bacteroidetes (16.2%), Fusobacteria (11.5%), the Actinobacteria (5.9%) and Proteobacteria (5.3%), which has the largest number of Firmicutes. Clostridiales was the most diverse bacterial order in Firmicutes, but Streptococcus, with 50% of all OTUs, was the most diverse bacterial genus in Clostridiales. [Conclusion] The findings of this study will facilitate the next step in understanding the complex phylogenetic diversity of the microbial communities in the intestinal tract of mink.