锁掷酵母油中营养成分的分离和鉴定

Separation and identification of the main nutriments in Sporidiobolus pararoseus oil

摘要:

【目的】研究一种新来源的微生物油脂的主要组成。【方法】对锁掷酵母(Sporidiobolus pararoseus)油中的主要功能性成分用两次硅胶柱层析进行分离纯化，得到9种物质。对这9种物质用薄板层析法、液相色谱洗脱和质谱法进行定量和定性分析。【结果】这9种物质为角鲨烯、β-胡萝卜素、γ-胡萝卜素、麦角固醇酯、红酵母烯、三甘酯、游离脂肪酸、麦角固醇和红酵母红素。其中麦角固醇的总浓度为3.2 g/kg油，16.7%的麦角固醇以麦角固醇酯的形式存在，角鲨烯的含量为1.25 g/kg油，类胡萝卜素含量为0.4 g/kg油。分析了麦角固醇酯和三甘酯中的脂肪酸组成，锁掷酵母油含有约73%的油酸，它可以作为良好的单不饱和脂肪酸来源。【结论】锁掷酵母油可能成为功能性油脂的来源。

Abstract:

[Objective] A new source of oil, produced by microorganism, was studied in the article. [Methods] Nine kinds of the main compositions were separated by twice slica column chromatography from the oil produced by Sporidiobolus pararoseus. The compositions were identified and quantized by thin layer chromatography, HPLC and HPLC-MS. [Results] The predominant fractions were squalene, β-carotene, γ-carotene, ergosterol esters, torulene, triglyceride, free fatty acids, ergosterol and torularhodin. The concentration of total ergosterol was about 3.2 g/kg in the Sporidiobolus pararoseus oil, and 16.7 % of the ergosterol exists in the form of ergosterol ester. The concentration of squalene in the oil was about 1.25 g/kg. The concentration of carotenoids in the oil was approximately 0.4 g/kg. Besides, the fatty acids compositions of ergosterol esters and triglyceride were also studied. There was about 73% oleic acid in the oil. [Conclusion] These results showed that S. pararoseus oil can be a good source of functional oil.