雷公藤植物内生Micromonospora sp. M66生产的一组吲哚生物碱

Indole alkaloids from endophytic Micromonospora sp. M66 of Tripterygium wilfordii Hook. f.

摘要:

【目的】研究稀有放线菌——雷公藤内生小单孢菌(Micromonospora sp. M66)的次级代谢产物，为微生物药物或农用生物制剂开发提供结构多样的化合物资源。【方法】利用薄层层析、正(反)相硅胶柱层析、凝胶层析、液相色谱等技术对M66菌株中次级代谢产物进行分离纯化，利用波谱技术对化合物进行结构鉴定。【结果】最终分离纯化了7个单体化合物，结合质谱与核磁技术对这7个化合物进行了结构解析和鉴定，它们属于一组吲哚生物碱。化合物2是重要的植物生长调节剂，化合物3对淋巴细胞性白血病细胞P388、枯草芽孢杆菌和酿酒酵母的增殖有抑制作用，化合物6对金黄色葡萄球菌有很好的抑制作用。【结论】化合物3-7首次从小单胞菌中鉴定出来，表明该小单孢菌具有较强的利用吲哚或色氨酸合成次级代谢产物的能力和挖掘生物碱类药物的潜力。

Abstract:

[Objective] We studied secondary metabolites of endophytic Micromonospora sp. M66 of Tripterygium wilfordii Hook. f., a rare actinomycete, provide structurally diverse compounds for microbial drugs and pesticides development. [Methods] Combining thin-layer chromatography, silica gel column chromatography, gel chromatography and semi-preparative high-performance liquid chromatography, we purified the secondary metabolites from Micromonospora sp. M66. By using the spectroscopic techniques, we elucidated their chemical structures. [Results] Seven compounds were purified and their chemical structures were elucidated through spectroscopic analysis including mass spectroscopies and nuclear magnetic resonance (NMR) spectroscopies. These 7 compounds were a set of indole alkaloids derived from tryptophan or indole. Compound 2 was an important plant growth regulator. Compound 3 showed a notable growth inhibition of lymphocytic leukemia cell P388, Bacillus subtillis and Saccharomyces cerevisiae, whereas compound 6 exhibited a significant inhibiting activity against Staphylococcus aureus. [Conclusion] Compounds from the bacteria of the genus of Micromonospora produce indole alkaloid-like secondary metabolites by using indole or tryptophan as a starting material and have potential as new alkaloid-like drugs.