Abstract:Massive open online course (MOOCs) and micro-lecture were products of the information age, and also were the developing direction of higher education in the future. In this paper, MOOCs and micro-lecture were applied to the reform and exploration of fermentation engineering course at the localized application-oriented universities and to supplement and extend the classroom teaching, they were new models for both taking into account the classroom teaching and the personalized learning, and will bring out the best in students’ personalized learning. For more than a year of teaching reform and exploration, it indicated that the teaching reform of MOOCs and micro-lecture had been approved by the students and the teachers and it indeed conducive to improving the students’ learning performance. In particular, the promotion of practice results achieve to a significant level (P<0.05), it was consistent with the purpose of training applied talents, and it was worth learning for localized application-oriented universities learning.