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嵌合O/GSLX/CHN/2010株S片段的基因工程口蹄疫病毒的生物学特性
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基金项目:

兰州市科技计划项目(No. 2012-2-72);公益性行业(农业)科研专项项目(No. 201103008)


Characterization of chimeric foot-and-mouth disease viruses bearing the S-fragment of O/GSLX/CHN/2010
Author:
  • JIANG Shao-Dong

    JIANG Shao-Dong

    Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences, State Key Laboratory of Veterinary Etiological Biology, OIE/China National Foot-and-Mouth Disease Reference Laboratory,Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou, Gansu 730046, China
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  • LI Ping-Hua

    LI Ping-Hua

    Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences, State Key Laboratory of Veterinary Etiological Biology, OIE/China National Foot-and-Mouth Disease Reference Laboratory,Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou, Gansu 730046, China
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  • SUN Pu

    SUN Pu

    Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences, State Key Laboratory of Veterinary Etiological Biology, OIE/China National Foot-and-Mouth Disease Reference Laboratory,Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou, Gansu 730046, China
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  • MA Xue-Qing

    MA Xue-Qing

    Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences, State Key Laboratory of Veterinary Etiological Biology, OIE/China National Foot-and-Mouth Disease Reference Laboratory,Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou, Gansu 730046, China
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  • BAI Xing-Wen

    BAI Xing-Wen

    Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences, State Key Laboratory of Veterinary Etiological Biology, OIE/China National Foot-and-Mouth Disease Reference Laboratory,Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou, Gansu 730046, China
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  • BAO Hui-Fang

    BAO Hui-Fang

    Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences, State Key Laboratory of Veterinary Etiological Biology, OIE/China National Foot-and-Mouth Disease Reference Laboratory,Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou, Gansu 730046, China
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  • LU Zeng-Jun

    LU Zeng-Jun

    Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences, State Key Laboratory of Veterinary Etiological Biology, OIE/China National Foot-and-Mouth Disease Reference Laboratory,Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou, Gansu 730046, China
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  • CAO Yi-Mei

    CAO Yi-Mei

    Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences, State Key Laboratory of Veterinary Etiological Biology, OIE/China National Foot-and-Mouth Disease Reference Laboratory,Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou, Gansu 730046, China
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  • FU Yuan-Fang

    FU Yuan-Fang

    Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences, State Key Laboratory of Veterinary Etiological Biology, OIE/China National Foot-and-Mouth Disease Reference Laboratory,Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou, Gansu 730046, China
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  • LI Dong

    LI Dong

    Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences, State Key Laboratory of Veterinary Etiological Biology, OIE/China National Foot-and-Mouth Disease Reference Laboratory,Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou, Gansu 730046, China
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  • CHEN Ying-Li

    CHEN Ying-Li

    Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences, State Key Laboratory of Veterinary Etiological Biology, OIE/China National Foot-and-Mouth Disease Reference Laboratory,Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou, Gansu 730046, China
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  • LIU Zai-Xin

    LIU Zai-Xin

    Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences, State Key Laboratory of Veterinary Etiological Biology, OIE/China National Foot-and-Mouth Disease Reference Laboratory,Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou, Gansu 730046, China
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    摘要:

    【目的】研究嵌合口蹄疫病毒(Foot-and-mouth disease virus,FMDV) O/GSLX/CHN/2010株S片段的基因工程病毒的生物学特性。【方法】运用反向遗传操作技术,将FMDV O/GSLX/CHN/2010的S片段嵌合进2株同谱系FMDV的感染性克隆骨架,拯救得到两株基因工程嵌合病毒rGDexc、rGZexc;进而从病毒复制和病毒对乳鼠的致病力方面,对嵌合病毒、亲本基因工程病毒以及FMDV O/GSLX/CHN/2010株进行了评价。【结果】FMDV O/GSLX/CHN/2010株复制较慢,对乳鼠致病力较差。嵌合病毒和亲本基因工程病毒的复制能力、对乳鼠的致病力明显高于FMDV O/GSLX/CHN/2010株。【结论】研究表明FMDV O/GSLX/CHN/2010的S片段不能独自决定该病毒较差的复制能力和较弱的乳鼠致病力。这一发现为进一步研究O/GSLX/CHN/2010的致弱机制以及S片段对病毒生物学特性的影响奠定了基础。

    Abstract:

    [Objective] We studied the characteristics of chimeric foot-and-mouth disease viruses (FMDVs) bearing the S-fragment of O/GSLX/CHN/2010. [Methods] Based on 2 existing full-length infectious cDNA clones with the same lineage to O/GSLX/CHN/2010, 2 chimeric FMDVs bearing the S-fragment of O/GSLX/CHN/2010 were constructed and rescued by reverse genetic manipulation. Virus replication and virulence in suckling mice of the chimeric viruses, parent genetically engineering viruses and FMDV O/GSLX/CHN/2010 were evaluated. [Results] FMDV O/GSLX/CHN/2010 replicated slowly and its virulence in suckling mice was weak. Whether in virus replication or virulence in suckling mice, the chimeric viruses and their parent genetically engineering viruses were better than FMDV O/GSLX/CHN/2010 significantly. [Conclusion] S-fragment of FMDV O/GSLX/CHN/2010 cannot determine the weak phenotype of O/GSLX/CHN/2010 alone, which lay the foundation for further studies of the attenuation mechanism of O/GSLX/CHN/2010 and the influence of S-fragment on FMDV characteristics.

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姜韶东,李平花,孙普,马雪青,白兴文,包慧芳,卢曾军,曹轶梅,付元芳,李冬,陈应理,刘在新. 嵌合O/GSLX/CHN/2010株S片段的基因工程口蹄疫病毒的生物学特性[J]. 微生物学通报, 2015, 42(12): 2396-2406

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  • 在线发布日期: 2015-12-09
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