嵌合O/GSLX/CHN/2010株S片段的基因工程口蹄疫病毒的生物学特性

Characterization of chimeric foot-and-mouth disease viruses bearing the S-fragment of O/GSLX/CHN/2010

摘要:

【目的】研究嵌合口蹄疫病毒(Foot-and-mouth disease virus，FMDV) O/GSLX/CHN/2010株S片段的基因工程病毒的生物学特性。【方法】运用反向遗传操作技术，将FMDV O/GSLX/CHN/2010的S片段嵌合进2株同谱系FMDV的感染性克隆骨架，拯救得到两株基因工程嵌合病毒rGDexc、rGZexc；进而从病毒复制和病毒对乳鼠的致病力方面，对嵌合病毒、亲本基因工程病毒以及FMDV O/GSLX/CHN/2010株进行了评价。【结果】FMDV O/GSLX/CHN/2010株复制较慢，对乳鼠致病力较差。嵌合病毒和亲本基因工程病毒的复制能力、对乳鼠的致病力明显高于FMDV O/GSLX/CHN/2010株。【结论】研究表明FMDV O/GSLX/CHN/2010的S片段不能独自决定该病毒较差的复制能力和较弱的乳鼠致病力。这一发现为进一步研究O/GSLX/CHN/2010的致弱机制以及S片段对病毒生物学特性的影响奠定了基础。

Abstract:

[Objective] We studied the characteristics of chimeric foot-and-mouth disease viruses (FMDVs) bearing the S-fragment of O/GSLX/CHN/2010. [Methods] Based on 2 existing full-length infectious cDNA clones with the same lineage to O/GSLX/CHN/2010, 2 chimeric FMDVs bearing the S-fragment of O/GSLX/CHN/2010 were constructed and rescued by reverse genetic manipulation. Virus replication and virulence in suckling mice of the chimeric viruses, parent genetically engineering viruses and FMDV O/GSLX/CHN/2010 were evaluated. [Results] FMDV O/GSLX/CHN/2010 replicated slowly and its virulence in suckling mice was weak. Whether in virus replication or virulence in suckling mice, the chimeric viruses and their parent genetically engineering viruses were better than FMDV O/GSLX/CHN/2010 significantly. [Conclusion] S-fragment of FMDV O/GSLX/CHN/2010 cannot determine the weak phenotype of O/GSLX/CHN/2010 alone, which lay the foundation for further studies of the attenuation mechanism of O/GSLX/CHN/2010 and the influence of S-fragment on FMDV characteristics.