DNA克隆和组装技术研究进展

Progress in DNA cloning and assembly techniques

摘要:

DNA克隆和组装技术是重要的分子生物学工具。近年来，随着合成生物学的飞速发展，对大片段DNA元件的快速有效组装就显得尤为关键。同时，各种DNA克隆和组装技术也竞相发展起来。通过对基于非典型酶切连接、PCR、同源重组、单链退火拼接等原理发展起来的各种DNA克隆和组装技术进行综述，为合成生物学的进一步发展提供有效的操作工具。

关键词:DNA克隆和组装，非典型酶切连接，PCR，同源重组，单链退火拼接

Abstract:

DNA cloning and assembly techniques are essential tools for molecular biology research. With the recent advances in synthetic biology, efficient and fast assembly of large DNA including a number of genes is becoming more and more important. Meanwhile, a variety of DNA assembly methods are also developed very quickly. In this paper, various DNA assembly methods based on atypical enzyme digestion and ligation, PCR, homologous recombination, single strand annealing and splicing are summarized for providing effective technique tools for the further development of synthetic biology.

Key words:DNA cloning and assembly, Atypical enzyme digestion and ligation, PCR, Homologous recombination, Single strand annealing and splicing