一株具有广谱抗菌活性炭样小单孢菌的全基因组序列测定

Whole genome sequencing of a Micromonospora carbonacea strain with broad-spectrum antimicrobial activity

摘要:

【目的】炭样小单孢菌JXNU-1是一株具有广谱抗菌活性的放线菌，研究揭示该菌的基因组序列信息。【方法】采用高通量测序技术对炭样小单孢菌JXNU-1的基因组DNA测序，利用SOAPdenovo软件组装，人工PCR修补基因组部分缺口，然后进行生物信息学分析。【结果】对炭样小单孢菌JXNU-1的全基因组序列进行了测定和注释，得到基因组精细图，相关序列已提交GenBank，获得登录号为JXSX00000000。【结论】研究为揭示炭样小单孢菌JXNU-1抗生素产生机制及其抗菌机理提供了基础数据，对进一步研发其抗生素具有重要的理论意义和巨大的应用价值。

关键词:炭样小单孢菌，全基因组测序，生物合成基因簇，抗菌活性

Abstract:

[Objective] Micromonospora carbonacea JXNU-1 is an actinomycete with broad-spectrum antimicrobial activity. This study aims to reveal its genomic sequence information. [Methods] The genomic DNA from M. carbonacea JXNU-1 was sequenced by Sanger technology using Illumina MiSeq and Illumina HiSeq 2500 sequencers. The genome was assembled by using SOAPdenovo software; multiplex PCR was used to close the gaps, and the genome sequence information was analyzed by bioinformatics methods. [Results] The whole-genomic DNA of M. carbonacea JXNU-1 was sequenced and annotated, and a fine genome map of M. carbonacea JXNU-1 was completed. This Whole Genome Shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession JXSX00000000. [Conclusion] This study provides the basis to explore biosynthesis of antimicrobials from M. carbonacea JXNU-1.

Key words:Micromonospora carbonacea, Whole-genome sequencing, Biosynthetic gene cluster, Antimicrobial activity