Abstract:[Objective] Identification specimen Dxhir140901 which parasitized on Lepidopterous larvae. [Methods] The comparison of morphological characteristics and the analysis of phylogenetic tree and the evolution network based on internal transcribed spacer (ITS1-5.8S-ITS2rDNA) sequence were used for identification of this specimen. [Results] Morphological observation: morphological characteristics of the strains Gzuifr-hir140901 was similar to Hirsutella Pat. It has two types of phialides, A-phialides, column, (1.8?6.3) μm×1.8 μm. B-phialides, columnar base, awl-shape and tapers, width of base 3?3.8 μm, length 21?63 μm, width of neck 1.8?2.0 μm, Phialides formed directly from the end of mycelium. Conidia orange segments or oval, (8.1?10.8) μm×(2.7?5.4) μm, mucus thick 1.8?2.7 μm. From the results of the phylogenetic analysis we can find that the Gzuifr-hir140901 strains and Ophiocordyceps macroacicularis S. Ban, T. Sakane & Nakagiriin belong to the same clade with a 98% approval rating. Branching sequence analysis supports the same results. [Conclusion] The sample Dxhir140901 and strains Gzuifr-hir140901 was a anamorph of O. macroacicularis which is a new record from China.
