中国野生发光真菌新记录种Neonothopanus nambi的分离、鉴定及其形态观察

Isolation, identification and characterization of Neonothopanus nambi (Basidiomycota, Fungi), a new record from China

摘要:

【目的】在中国福建地区采集分离一株野生发光真菌，菌种编号为MRC-lf3，对其进行形态观察及分子鉴定以确定其分类地位，并观察其整个生活史的形态特征。【方法】采用组织分离技术获得野生发光真菌纯培养物，结合形态学及ITS进化分析确定该菌的分类地位，应用单反相机记录子实体不同生长时期的形态特征及发光情况。【结果】该发光真菌的菌丝、子实体、孢子的形态均与Neonothopanus nambi最为相似，ITS序列的进化分析结果也支持该发光真菌为Neonothopanus nambi。其子实体颜色深浅和发光强度均受到光照强度的影响，光照可使子实体颜色变暗、发光强度减弱。【结论】从福建省福州市采集分离获得一株大型野生发光真菌，经鉴定该菌为Neonothopanus nambi，是中国首次发现的Neonothopanus属真菌，该发现也将中国大陆的野生发光真菌总数增加至8种。

Abstract:

[Objective] A luminous Basidiomycete, designated MRC-lf3, was isolated from Fujian, China, and its taxonomic status was confirmed, and its life cycle was recorded. [Methods] The mycelial pure culture was obtained by tissue isolation method; morphological characteristics and the phylogenetic analysis of ITS rDNA sequence were used to analyze the taxonomic status of this fungus; the morphological and luminous characteristics were recorded by Digital Single Lens Reflex. [Results] Both morphological characteristics and ITS rDNA phylogenetic analysis results demonstrated that the strain MRC-lf3 was Neonothopanus nambi. Our study also finding that both ability to emit light and color of the fruiting body could be changed by different illumination intensity. [Conclusion] We isolated the luminous fungal strain from Fujian China, which was identified as Neonothopanus nambi, this is the ?rst record of genus Neonothopanus in China. This new record of Neonothopanus nambi brings the total number of luminous fungi in the Chinese Mainland to 8 species.