激光共聚焦扫描显微镜在抗菌机理研究中的应用

Applications of confocal laser scanning microscope in the research of antimicrobial mechanism

摘要:

激光共聚焦扫描显微镜采用激光光源、共聚焦技术和点扫描技术，使其分辨力较传统光学显微镜大为提高。与其他的生物学技术相配合，可定性、定量、定位地检测组织细胞内的多种生化成分。它具有的活细胞动态监测、断层扫描及三维图像重建等功能，使其在抗菌机理研究、尤其是抗生物膜研究中得到大量的应用。本文就激光共聚焦扫描显微镜在抗菌剂作用位点，抗菌剂对微生物细胞膜，微生物生理代谢，以及微生物生物膜形成与结构的影响等研究中的应用做一综述。

Abstract:

Confocal laser scanning microscope (CLSM or LSCM) offers higher resolution than traditional optical microscopy due to use of laser illumination source, pinhole spatial filter and point scanning technology. In combination with other biological technologies, CLSM enables the qualitative and quantitative characterization of various biochemical ingredient in situ. As CLSM is a valuable tool for observing living cells, obtaining scan-images of thick specimens at various depths and three-dimensional reconstructions, it has great application in the research of antimicrobial and anti-biofilm. This paper concerns the applications of CLSM in the cellular distribution of microbicides and its effect on cell membrane, metabolic pathways, as well as the formation and structure of biofilm.