Abstract:The secretome is a group of proteins secreted by cells and/or tissues at a given time and under a given condition. Most phytopathogenic fungi can secrete many proteins and metabolites during the plant-fungi interaction, and the secreted proteins and metabolites play important roles at different infection stages of fungal penetration, colonization, and lesion formation. In this review, we summarized recent advances in biological functions of secreted proteins in fungal pathogenicity, the secretome of important plant pathogenic fungi, and bioinformatics prediction of fungal secretomes, which will help to understand the mechanisms involved in fungal pathogenesis.