terC基因对天蓝色链霉菌抗生素合成和菌丝体生长的影响

Effects of terC on antibiotics biosynthesis and mycelium development in Streptomyces coelicolor M145

摘要:

【目的】研究天蓝色链霉菌中terC (SCO2366)基因的功能。【方法】通过敲除天蓝色链霉菌中terC基因，检测其对放线紫红素合成和菌丝体生长的影响。【结果】敲除天蓝色链霉菌中的terC基因后，放线紫红素提前合成，同时菌丝体长度变短。【结论】terC在天蓝色链霉菌中对放线紫红素的合成有负调控作用，同时影响菌丝体生长发育。

Abstract:

[Objective] To study the function of terC (SCO2366) in Streptomyces coelicolor M145. [Methods] Through knocking out the terC in S. coelicolor M145, we detected its effects on actinorhodin biosynthesis and mycelium development. [Results] After knocking out the terC in S. coelicolor M145, the synthesis of actinorhodin began earlier than the wild type strain, and the length of mutant’s mycelium got shorter. [Conclusion] terC in S. coelicolor M145 has negative effect on actinorhodin biosynthesis, meanwhile affects mycelium development.