邳州银杏内生放线菌分离、筛选及活性菌株鉴定

Isolation, screening and preliminary identification of endophytic actinobacteria from Ginkgo biloba

摘要:

【目的】从银杏中分离、筛选得到具有抑菌作用的内生放线菌，为放线菌在生物防治上的应用提供新的菌种资源。【方法】采用组织贴片培养法进行分离，生长对峙法进行筛选。【结果】从银杏的根、茎、叶中分离得到98株、50株、8株内生放线菌(共计156株)，47株放线菌具有拮抗植物病原真菌活性。菌株KLBMP 5501抗菌活性最好且具有广谱性，基于形态特征、培养特征、生理生化特征和16S rRNA基因序列的相似性分析等多项分类特征表明，菌株KLBMP 5501是一株浅紫链霉菌(Streptomyces violascens)。【结论】筛选得到了具有应用潜力的高活性菌株，并进行了菌种鉴定。

Abstract:

[Objective] To isolate and screen actinomycetes with antimicrobial activity from Ginkgo biloba, and provide new resources for the application of actinomycetes in biological control. [Methods] The cultural method of plant tissue paste on medium were used to isolate actinomycetes. [Results] In total, we have isolated 156 endophytic actinomycetes from Ginkgo biloba. The fermentation broth of 47 strains could inhibit growth of the tested phant pathogenic fungi, and the strain KLBMP 5501 exhibited extensive antagonism on most of tested pathogens. Based on the morphological characteristics, cultural characteristics, physiological characteristics and 16S rRNA gene sequence similarity analysis, strain KLBMP 5501 was identified as Streptomyces violascens, a known species of Streptomyces. [Conclusion] Actinomycetes strains with high antimicrobial activity were obtained and showed potential for biological control of plant diseases. One of these strains, KLBMP 5501, was identified.