Abstract:[Objective] Identification and reporting two Aspergillus isolates as a new Chinese record species, i.e, A. germanicus. [Methods] Polyphasic studies using morphological characters and clamodulin gene, β-tubulin gene and rDNA ITS1-5.8S-ITS2 sequences. [Results] Based on the comparisions of morphological and molecular characters of the two Aspergillus isolates (AS3.15303 and AS3.15304, isolated from soil in Shandong Province, China) with the ex-type of A. germanicus (CBS 123887), the two Chinese isolates were identified as A. germanicus. [Conclusion] We confirmed that A. germanicus was a new Chinese record of Aspergillus section Usti.