一株产电菌Nitratireductor sp. WJ5-4的筛选及产电分析

Isolation and characterization of an electricity-producing strain Nitratireductor sp. WJ5-4

摘要:

【目的】从生物垃圾燃料电池阳极淋洗液中分离一株产电菌WJ5-4，研究其产电特性。【方法】根据菌株的形态、生理生化性质及16S rRNA基因测序分析确定其种属，以该菌株为产电菌，以生物垃圾为底物，构建微生物燃料电池(Microbial fuel cell，MFC)，研究在不同接种浓度和底物固含量条件下菌株的产电性能。【结果】菌株WJ5-4被初步鉴定属于Nitratireductor属，当接种量200 mL时可获得最大功率密度135.16 mW/m2、稳定电压370 mV和总有机碳(Total organic carbon，TOC)降解率41.46%。当底物固含量为23%时，可获得最大功率密度163.69 mW/m2、稳定电压434 mV和TOC降解率46.29%。【结论】WJ5-4菌能够利用较高固含量的生物垃圾产电，产电周期较长，为下一步微生物燃料电池处理生物垃圾提供科学依据。

Abstract:

[Objective] To isolate and obtain an electrochemically bacterium (exoelectrogen), strain WJ5-4, from anode leacheate of MFC using biowaste as substrate and to study its electrical characteristics. [Methods] The strain was identified by morphological observation, physiological and biochemical properties and 16S rDNA sequence analysis. MFC (microbial fuel cell) was constructed using strain WJ5-4 as exoelectrogen and using biowaste as substrate. The electrical properties of strain WJ5-4 was studied on different inoculation concentration and solid content. [Results] The strain WJ5-4 belonged to Nitratireductor, when inoculation concentration was 200 mL, the maximum power density was 135.16 mW/m2, stable working voltage was 370 mV and degradation of TOC (total organic carbon) was up to 41.46%. When solid content was 23%, power density was 163.69 mW/m2, the voltage was 434 mV, and degradation of TOC was up to 46.29%. [Conclusion] Strain WJ5-4 could produce electricity using higher solid content biowaste, and the electricity production cycle was longer. These results would provide reliable basis for further treatment of biowaste by the means of MFC.