Abstract:Talent cultivation mode in universities must fit with the cooperation of production, teaching and research. To cultivate the ability of actual application and professional analysis of students in application-oriented universities, the reform and innovation should be kept during the setting process of talent cultivation mode and construction of base for production-teaching-research for Biological Engineering specialty. Taking Xuzhou Insititute of Technology for example, this paper analyzed past education expeience in production-teaching-research and problems in talent cultivation mode, indicated that production-teaching-research for biological engineering specialty in application-oriented universities should be taken into practice.