Abstract:[Objective] This study chose healthy adult volunteers of Bai ethnic group at Yunnan Province as the research object, investigating the relationship between the structural intestinal flora and human health. [Methods] This study recruits 43 healthy adult volunteers of Bai ethnic group living in Kunming urban and Er Yuan rural county Dali Bai autonomous prefecture at Yunnan Province as the research object, analyzed the dominant bacterium of intestinal flora by specificity primer qualitative PCR and polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE), and measured the content of short chain fatty acids (SCFAs) in the feces. [Results] Results in present study showed that although the average of SCFAs content in Rural is higher than that of Urban, the difference was not significant. The qualitative PCR results showed that, for the diversity of Lactobacillus and Bifidobacterium, these two groups have obviously differences, Unweighted pair-group method with arithmetic means (UPGMA) based on optical density was performed on the base of 16S rRNA V3 region PCR-DGGE profile, showing samples were divided into two groups as urban and rural groups. We extracted the typical bands for further cloning and sequencing, and constructing phylogenetic tree diagram the results showed that the Bifidobacterium, Enterubacterium and Enterococcus have a high diversity, suggesting that it was predominant in the gut of healthy volunteers living in county of Dali bai autonomous prefecture Er Yuan Xian. [Conclusion] To sum up, the structural intestinal flora of Bai ethnic group living in urban of Kunming and Er Yuan rural county Dali Bai autonomous prefecture at Yunnan Province has the distinguishing trend, but the difference of diversity was not significant. The current study aimed to provide certain reference for exploring the relationship between healthy host and intestinal flora.
