3-苯氧基苯甲酸降解菌Sphingomonas sp. SC-1降解苯酚环境条件及其降解中间产物的研究

Study on the influence of environmental conditions and intermediates of phenol degradation by a 3-phenoxybenzoic acid degrading strain Sphingomonas sp. SC-1

摘要:

【目的】探究高效降解3-苯氧基苯甲酸(3-Phenoxybenzoic acid，3-PBA)的鞘氨醇单胞菌(Sphingomonas sp.) SC-1对苯酚的降解特性。【方法】采用HPLC测定微生物降解体系中苯酚残留量，考察环境条件对菌株SC-1降解苯酚的影响；分析不同培养时间苯酚降解体系混合样品的HPLC谱图，确定其降解中间产物。【结果】菌株SC-1能在基础盐培养基中以苯酚为唯一碳源和能源生长，在初始pH 7.0、30 °C条件下，24 h可完全降解100 mg/L苯酚；Cu2+、Ba2+、Mn2+等对其降解苯酚有不同程度的抑制作用；HPLC谱图分析，初步确定邻苯二酚是菌株SC-1降解苯酚的中间产物，且该菌株可在48 h内完全降解100 mg/L邻苯二酚。【结论】菌株SC-1对苯酚及邻苯二酚均有较强的降解能力，为完善3-PBA的降解途径及污染3-PBA或含酚废水或含酚农药残留的降解提供了数据参考。

Abstract:

[Objective] The degradation characteristics of phenol by a 3-phenoxybenzoic acid degrading strain-Sphingomonas sp. SC-1 were studied. [Methods] The influence factors of phenol degradation were investigated by determining the concentrations of phenol in microbial degradation systems by HPLC. We analyzed the intermediate products of phenol by analyzing the HPLC chromatograms of the mixed samples taken from phenol degradation systems at different time. [Results] The results showed that SC-1 could grow well by using phenol as sole carbon source in mineral basal medium. Strain SC-1 could degrade phenol entirely within 24 hours while cultured in the medium (pH 7.0) containing 100 mg/L phenol at 30 °C. The strain’s growth and phenol degradation were inhibited when the training systems were treated with some heavy metal ions such as Cu2+, Ba2+, Mn2+, etc. Catechol was an intermediate product of phenol and strain SC-1 could degrade catechol (100 mg/L) within 48 hours. [Conclusion] This research indicated that strain SC-1 could degrade phenol and its intermediate product catechol effectively, and this work could provide data reference for the thorough degradation of 3-phenoxybenzoic acid and the degradation of phenol containing wastewater or phenol containing pesticide residues.