植物病原丝状真菌G蛋白偶联受体的研究进展

Advance in functional research of G protein-coupled receptors in phytopathogenic filamentous fungi

摘要:

通过对丝状真菌G蛋白偶联受体(GPCR)的结构、分类以及功能方面进行综述，以期明确丝状真菌与其他模式生物GPCR之间的关系。基于已报道的模式生物及丝状真菌等不同生物中的GPCR，通过SMART保守结构域分析，以及利用Clustal X、MEGA等软件对上述GPCR进行遗传关系分析。明确丝状真菌典型GPCR具有七跨膜结构域，新型GPCR则含有PIPK、RGS等保守结构域，明确不同学者对于GPCR的分类情况，以及新型GPCR所具有的特殊功能，明确模式生物GPCR、丝状真菌GPCR分别各自聚类。丝状真菌中GPCR的数量较模式生物少，不同分类单元中真菌之间GPCR的数量也不尽相同，同时，丝状真菌GPCR除具有典型的七跨膜结构域外，还含有一些其他保守的结构域，上述研究为进一步开展其功能研究提供重要的理论基础。

Abstract:

To determine the structure, classification and functional aspects of G protein-coupled receptor (GPCR) in filamentous fungi, and to determine the relationship of GPCR between filamentous fungi and other model organisms. Based on the reported GPCR in the model organisms and filamentous fungi, the conserved domain was analyzed by SMART web service, and the genetic relationship between filamentous fungi and other model organisms was identified by Clustal X and MEGA software. There were seven transmembrane domains of typical GPCR and novel GPCR with PIPK, RGS and other conserved domains in filamentous fungi. The classification of GPCR from different scholars was identificated, as well as novel GPCR has special function. The model organisms GPCR and filamentous fungi GPCR can be grouped into each category. The number of GPCR of filamentous fungi was less than that of the model organism, and the number of GPCR was not the same among fungal taxa. GPCR in filamentous fungi contains a number of other conserved domains in addition to a typical seven transmembrane, which provide important theoretical basis for further study on its function.