线虫共生致病杆菌PacYellow的吲哚抗生素

Indole antibiotics from entomopathogenic bacterium Xenorhabdus bovienii PacYellow

摘要:

【目的】研究特殊生境的微生物——嗜线虫致病杆菌(Xenorhabdus bovienii PacYellow)的次级代谢产物，为微生物药物或农用抗生素开发提供结构多样的化合物。【方法】通过16S rRNA基因比对鉴定菌株；利用薄层层析、硅胶柱层析、凝胶层析、液相制备等技术进行分离纯化；利用质谱、红外光谱与核磁共振等光谱技术鉴定化合物结构。【结果】PacYellow菌株被鉴定属于致病杆菌属bovienii种。从该菌株中分离纯化了4个单体化合物，它们的结构被鉴定为吲哚类衍生物，可能源于色氨酸与缬氨酸或异亮氨酸的缩合产物。【结论】从嗜线虫致病杆菌——PacYellow菌株中分离鉴定了4个吲哚生物碱类抗生素，它们具有中等到高的抗菌活性。

Abstract:

[Objective] To study secondary metabolites of special eco-environmental microorganism— Xenorhabdus bovienii PacYellow, and to provide structurally diverse compounds for microbial drug/pesticide development. [Methods] The strain PacYellow was identified by comparison of 16S rRna gene. The secondary metabolites were purified by thin-layer chromatography, silica gel column chromatography, gel chromatography, and semi-preparative high-performance liquid chromatography. The structures were elucidated by using spectroscopic technologies including nuclear magnetic resonance (NMR), infrared spectroscopy (IR) and mass spectroscopy (MS). [Results] The strain PacYellow was identified as a strain of Xenorhabdus bovienii. Four compounds were purified and their chemical structures were elucidated as indole derivatives that are probably biosynthesized from tryptophan and valine or isoleucine. [Conclusion] Four indole derivatives were identified from entomopathogenic bacterium Xenorhabdus bovienii PacYellow.