深圳海域6株破囊壶菌的生长特性及油脂成分分析

Growth features and fatty acid analysis of six thraustochytrid strains from Shenzhen coastal seawater

摘要:

【目的】从深圳海域分离得到6株破囊壶菌，对其基本形态特征、生活史和油脂含量等进行研究，开发其应用潜力。【方法】使用松花粉垂钓法对破囊壶菌进行分离，通过18S rRNA基因测序的方法对破囊壶菌进行鉴定，用显微镜观察其基本形态特征，通过使用尼罗红(Nile Red)染色法对油脂含量进行定性检测，并用GC-MS分析菌株的油脂含量和组成情况。【结果】18S rRNA基因鉴定其属于Aurantiochytrium sp.、Schizochytrium sp.和Thraustochytrium sp.三个属。破囊壶菌的脂肪酸主要成分为十六碳饱和脂肪酸和二十二碳六烯酸(DHA)，其中Mn11和Mn15的饱和脂肪酸含量达到总脂肪酸含量的70%以上，Mn16和Sw7的DHA产量分别达到1.29 g/L和1.26 g/L。【结论】Mn11和Mn15菌株适合用于生物柴油的生产，Mn16和Sw7是DHA发酵生产的潜力菌株。

Abstract:

[Objective] Identified six thraustochytrid strains isolated from Shenzhen costal seawater and sediment. Morphological characteristics, life cycle and fatty acid profiles of these strains were studied. [Methods] Thraustochytrid strains were isolated using pine pollen as bait and identified based on 18S rRNA gene sequences. Morphological characteristics were observed using microscope. Nile red staining was used to qualitatively analyze oil content. While GC-MS were used to analyze specific fatty acid profile. [Results] Analysis in 18S rRNA gene revealed that they belong to three genera Aurantiochytrium sp., Schizochytrium sp. and Thraustochytrium sp.. Among these strains, Mn11 and Mn15 were found to produce high level of saturated fatty acid, which is more than 70% total fatty acid. While Mn16 and Sw7 showed high capacity of DHA production with 1.29 g/L and 1.26 g/L DHA yield. [Conclusion] Mn11 and Mn15 strains were suitable for biodiesel production while Mn16 and Sw7 were promising DHA producing strains.