高通量细菌鉴定方法研究进展

The research progress of high-throughput bacterial species identification

摘要:

高通量细菌鉴定是微生物领域的重要研究课题，对于疾病诊断和环境监测具有重要意义。相比传统的表型鉴定方法，分子遗传学鉴定方法具有稳定性高、检测周期短以及成本较低等特点，成为了主流的鉴定方法。特别是下一代DNA测序技术、核酸分子检测基础上的细菌检测芯片、质谱技术基础上的蛋白质图谱分析为高通量、快速、准确乃至定量的细菌鉴定提供了可行性方案。

Abstract:

High-throughput bacterial species identification is an important research topic in the area of microbiology, which plays a pivotal role in the disease diagnosis and environmental monitoring. Compared to the traditional phenotypic identification methods, molecular methods have the features of higher stability, shorter detection period and lower cost, which are becoming the main trend in the bacterial species identification. Especially, next generation sequencing technique, the detection chips based on nucleic acid identification, the protein profile analyses based on mass spectrometry provide the valid means for high-throughput, fast, accurate and quantitative bacterial species identification.