再接种内生真菌对葡萄叶内生真菌群落结构的影响

Effects on folia endogenous flora structure of grape after re-inoculation with different endophytic fungi

摘要:

【目的】探讨回接内生真菌对葡萄叶内生真菌群落结构的影响。【方法】将葡萄叶中分离到的8株内生真菌回接到健康生长的玫瑰蜜葡萄叶片上，同时设置管理上喷施农药与未喷施农药两组处理，3个月后分析不同真菌菌剂处理后葡萄叶片内生真菌的分离率、优势度和多样性指数。【结果】5种菌剂的处理(Xylaria sp.、Nigrospora sp.、Alternaria sp. 1、Alternaria sp. 2和Colletotrichum sp.)在处理后的叶片中分离到了较多数量的接种菌。不同菌剂处理后葡萄叶片菌群结构差异显著。【结论】与对照处理的葡萄相比，菌剂的处理增加了叶内生真菌的分离率，但显著降低了内生真菌的多样性指数；发现喷施农药组叶片内生真菌的分离率较低，但多样性相对较高。

Abstract:

[Objective] The research is to study the effects of endophytic fungi re-inoculation on folia endogenous flora structure of grape. [Methods] Eight strains of endophytic fungi which isolated from wine grape leaves were re-inoculated to the healthy leaves of grapevine (variety: Rose-honey), and the effects on the folia endophytic fungi structure of grape were analyzed. Meanwhile, effects differences on the endophytic fungi structure to both groups of grapevine with and without pesticide after endophytic fungi re-inoculation, were also studied. We describe the folia endophytic fungi structure by using the isolation rate, dominance index and diversity index three months after re-inoculation. [Results] The results indicated that among all the treatments, five strains of the inoculated endophytic fungi (Xylaria sp., Nigrospora sp., Alternaria sp. 1, Alternaria sp. 2 and Colletotrichum sp.) can be relatively higher frequently isolated. Significant differences of folia endophytic fungi structures were found between one fungus re-inoculation and another. [Conclusion] Compared with the controls, fungi re-inoculation can increase the isolation rate, but decrease the diversity index significantly of folia endophytes. And the using of pesticide could decrease the isolation rate, but shares a higher diversity of folia endophytic fungi, compared with that of the pesticide free group.