酸解羽毛粉代替蛋白胨研制新型细菌培养基

Using hydrolyzed feather powder to replace tryptone for producing a novel bacterial medium

摘要:

【目的】从工厂下脚料——酸解羽毛粉氨基酸含量高且种类丰富出发，开发出低成本新型细菌培养基，以期资源化该类废弃物。【方法】将酸解羽毛粉代替常用细菌培养基(LB培养基)中的蛋白胨，进行细菌液体发酵试验，比浊法在波长600 nm处比色测定菌液的吸光值，可培养计数法监测细菌数量。【结果】以LB培养基为对照，酸解羽毛粉完全代替蛋白胨液体发酵供试菌株时，菌株的生物量与对照无显著性差异或显著高于对照。培养模式菌株大肠杆菌和枯草芽孢杆菌24 h后，与对照相比，生物量分别增加了21.59%和27.83%。菌株生长曲线表明，生长初期，菌株在新型培养基中生长稍延迟，但含酸解羽毛粉培养基能延长枯草芽孢杆菌的对数生长期，并且两菌株到达稳定期时的生物量均高于对照。可培养计数法结果同样表明，含酸解羽毛粉培养基所培养活菌数量与对照(LB培养基)相比，差异不显著。【结论】用酸解羽毛粉代替LB培养基中蛋白胨进行细菌培养是可行的，可以大大降低生产成本。

Abstract:

[Objective] In this study, since hydrolyzed feather powder is a kind of good nitrogen-containing material rich in a wide variety of amino acids, it is used to replace the tryptone, a common component in Luria-Bertani medium for cultivating bacteria, thus to develop a novel bacterial culture medium and recycle the waste resource. [Methods] Absorbance at 600 nm of the fermentation broths detected by turbidimetry and culture-dependent method were used to analyze the effect of media on cell growth. [Results] Compared to the LB medium, no significant difference or more abundance in the growth of the tested bacteria was observed when peptone was completely replaced by hydrolyzed feather powder and the biomass of type strains (Escherichia coli and Bacillus subtilis) tested, increased by 21.59% and 27.83%, respectively. Results of bacteria-growth curves showed that a bit delay at the initial phase and an extended logarithmic phase of the tested strains were observed in the novel medium. However, the biomasses of the strains tested at stable phase in the novel medium were higher than those in the control. Meanwhile, results from culture-dependent method also showed that no significant differences for the number of type strains were observed between the two media. [Conclusion] In conclusion, the hydrolyzed feather powder was able to replace tryptone to create a novel low-cost but high-quality medium.