1. South China University of Technology, Guangzhou, Guangdong 510641, China; 2. The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Institute of Respiratory Disease, State Key Laboratory of Respiratory Disease, Guangzhou, Guangdong 510120, China 在期刊界中查找 在百度中查找 在本站中查找
2. The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Institute of Respiratory Disease, State Key Laboratory of Respiratory Disease, Guangzhou, Guangdong 510120, China 在期刊界中查找 在百度中查找 在本站中查找
2. The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Institute of Respiratory Disease, State Key Laboratory of Respiratory Disease, Guangzhou, Guangdong 510120, China 在期刊界中查找 在百度中查找 在本站中查找
[Objective] To study the infection by Legionella pneumophila and the relative biological activities between bacteria and host cells in real time. [Methods] We constructed a strain of L. pneumophila that can stably express green fluorescent protein by method of gene knockout and retro-complementation, and established an infection model with murine Raw264.7 macrophage. [Results] The recombinant strain was applied in cell infection successfully. The entire process of infection can be observed in real time by fluorescence microscope, including the variety of bacterial cell morphology, intracellular proliferation, and lysing the host cells. [Conclusion] Our result provides a new mean to study the relationship between L. pneumophila and infected cells, the preparation of some models related to the drugs, and the drug screening and the mechanism of drug resistance.
