侧孢短芽孢杆菌AMCC 100017的分离鉴定及其生防潜力

Isolation, identification and the biocontrol potential of a strain of Brevibacillus laterosporus

摘要:

【目的】从土壤中分离、筛选和鉴定具有抑制病原真菌活性等生防效果的菌株，为进一步开发利用具有良好定殖能力的生防菌株奠定基础。【方法】利用对峙试验筛选拮抗菌并评价其拮抗性能，根据其形态特征、生理生化特性、16S rRNA基因序列测定及GenBank序列相似性分析进行分离菌株的分类鉴定，并通过福林酚法测定该菌株的蛋白酶活力。【结果】从山东泰安各种类型土壤中分离得到侧孢短芽孢杆菌(Brevibacillus laterosporus)，保藏编号为AMCC 100017。平板对峙试验结果表明该菌株对多种植物病原真菌均有较强的拮抗作用，尤其是对镰刀菌属致病菌拮抗效果明显。另外，本试验还初步验证该菌能产生较高活性的胞外蛋白酶。【结论】侧孢短芽孢杆菌AMCC 100017在作物真菌病害生物防治方面，有较好的开发和利用潜力，并可望应用于线虫生物防治。

Abstract:

[Objective] In order to lay the foundation for the further development and utilization of biocontrol strains with good colonization ability, bacterial strains with antifungal activity were isolated, screened and identified from soil. [Methods] Antagonistic experiment was used to screen the specific bacteria against pathogenic fungi and evaluate its inhibitory ability. The strain was classified and identified by morphological characteristics, physiological and biochemical characteristics, sequencing of 16S rRNA gene and analysis of sequences similarity in GenBank. Protease activity of the test strain was measured by Folin-Ciocalteu method. [Results] Brevibacillus laterosporus, preservation number AMCC 100017, was isolated from various kinds of?soil in Tai’an, Shandong province. Plate confrontation experiment indicated that the strain has strong antagonistic effect against various plant pathogenic fungi, especially Fusarium. In addition, it was also verified that the strain could produce highly reactive extracellular protease. [Conclusion] Brevibacillus laterosporus AMCC 100017 is a biocontrol strain wih the development and utilization potential against fungal diseases of crops and fruit trees, as well as the biological control of nematodes.