乙酸驱动条件下粪产碱杆菌Y5的碳氮共脱除特性

Simultaneous carbon and nitrogen removal of Alcaligenes faecalis Y5

摘要:

【目的】研究微生物的碳氮共脱除特性及其关键影响因素。【方法】以乙酸为唯一碳源分离获得的碳氮共脱除菌株Y5为模式菌株，分析菌株Y5的16S rRNA基因序列、碳源和氮源去除动力学，以及碳源种类、碳氮比(C/N)、溶解氧浓度(DO)、温度和pH等影响效果。【结果】菌株Y5归属于粪产碱杆菌。与葡萄糖及多种有机酸相比，菌株Y5在以乙酸为唯一碳源的条件下具有较高的TOC和NH4+-N去除速率。在好氧条件下，当起始TOC浓度为1 000 mg/L，氨氮浓度为110 mg/L时，菌株Y5的NH4+-N、TOC和总氮(TN)去除率分别达99.54%、92.95%和86.55%，最大NH4+-N、TOC和TN去除速率分别为903.58、505.81和406.03 mg/(L·d)。【结论】粪产碱杆菌Y5在以乙酸为唯一碳源的条件下具有较强的碳氮共脱除能力，其最佳反应条件为：C/N=10，pH 7?8，溶氧>6.20 mg/L，反应温度为30 °C。

关键词:异养氨氧化，碳氮共脱除，粪产碱杆菌Y5

Abstract:

[Objective] Understanding the characteristics of simultaneous carbon and nitrogen removal under different conditions. [Methods] We took strain Y5, which was isolated in the medium with sodium acetate as the sole carbon source and capable of removing carbon and nitrogen simultaneously, as the model microorganism in this study. The 16S rRNA gene sequence of strain Y5 was analyzed. The carbon and nitrogen removal dynamics of strain Y5, as well as the influencing factors such as carbon source, ratio of carbon to nitrogen (C/N), dissolved oxygen concentration (DO), temperature, and pH were investigated. [Results] Strain Y5 belonged to Alcaligenes faecalis. Compared with glucose and other organic acids, strain Y5 showed higher total organic carbon (TOC) and NH4+-N removal rates in the medium with sodium acetate as the sole carbon source. When the initial TOC and NH4+-N were 1 000 mg/L and 110 mg/L, the NH4+-N, TOC and TN removal rates were 99.54%, 92.95% and 86.55% after 48 hours aerobic incubation, respectively. [Conclusion] Alcaligenes faecalis Y5 had efficient carbon and nitrogen simultaneous removal ability when taking sodium acetate as the sole carbon source and its optimal conditions were C/N=10, pH 7?8, DO>6.20 mg/L, 30 °C.

Key words:Heterotrophic ammoxidation, Simultaneous carbon and nitrogen removal, Alcaligenes faecalis Y5