科微学术

微生物学通报

乳酸杆菌对感染大肠杆菌O157:H7小鼠肠道黏膜免疫的影响
CSTR:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

国家863计划项目(No. 2011AA100902,2012AA022108);食品安全与营养协同创新中心资助项目


Function of Lactobacillus on intestinal mucosal immunity of mice artificially infected with Escherichia coli O157:H7
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    【目的】分析小鼠在感染Escherichia coli O157:H7及补充嗜酸乳杆菌KLDS AD1和瑞士乳杆菌KLDS1.8701期间小肠黏膜中SIgA和细胞因子的变化规律,结合小鼠表象特征,探讨2株乳酸杆菌对小鼠腹泻的治疗效果。【方法】将小鼠分成4组,空白组、致病对照组、嗜酸乳杆菌组和瑞士乳杆菌组,对实验组小鼠连续7 d灌胃大肠杆菌致病后,再连续7 d分别灌胃2株乳酸杆菌,采集小鼠小肠利用ELISA法测得各组小鼠肠道组织中SIgA和4种细胞因子IL-2、IFN-γ、IL-4和IL-6的含量。【结果】瑞士乳杆菌可极显著提高感染大肠杆菌O157:H7小鼠的体重,嗜酸乳杆菌的效果较小;感染E. coli O157:H7后,SIgA、IL-2和 IFN-γ的含量在第3天达到最大值,第5天开始下降,而IL-4和IL-6在第5天达到最大值,第7天开始下降。补充嗜酸乳杆菌和瑞士乳杆菌后,SIgA和4种细胞因子的含量都迅速增加,并保持较高水平,与其他两组差异显著。【结论】嗜酸乳杆菌KLDS AD1和瑞士乳杆菌KLDS 1.8701都可通过增加细胞因子和SIgA的分泌增强肠道黏膜免疫,对小鼠腹泻有一定的缓解作用。

    Abstract:

    [Objective] The possible immunomodulatory and anti-infectious effects of Lactobacillus acidophilus KLDS AD1 and Lactobacillus helveticus KLDS 1.8701 were evaluated on the intestinal mucosal immunity in the rats artificially infected with Escherichia coli O157:H7. [Methods] An model animal trial was carried out on rats divided into four treatments, challenged treatment with E. coli O157:H7 for seven days, artificially infected animals treated with L. acidophilus or L. helveticus for another seven days. The intestinal tissue was taken for assaying the secretory immunoglobulin A (SIgA) in intestinal fluid and cytokines (IL-2, IL-4, IL-6, IFN-γ) production in small intestinal tissue by ELISA. [Results] L. helveticus KLDS 1.8701 significantly attenuated the pathogenic influence of E. coli O157:H7 indicated by the high weight gain, better than L. acidophilus KLDS AD1. After treatment with E. coli O157:H7, the levels of SIgA, IL-2 and IFN-γ increased and reached to the maximum value at the third days, decreased at the fifth day, then IL-4 and IL-6 reached to the maximum value and decreased at the seventh day. The levels of SIgA and all assayed cytokines were significantly increased after oral administration of L. acidophilus KLDS AD1 and L. helveticus KLDS 1.8701 at eighth day, and remained higher level than control and pathogenic groups. [Conclusion] L. acidophilus KLDS AD1 and L. helveticus KLDS 1.8701 improved the intestinal mucosal immune by stimulating cytokines and SIgA production, and confered enhanced protection against E. coli O157:H7 infection.

    参考文献
    相似文献
    引证文献
引用本文

王婷婷,王雪芹,霍贵成. 乳酸杆菌对感染大肠杆菌O157:H7小鼠肠道黏膜免疫的影响[J]. 微生物学通报, 2014, 41(10): 2082-2089

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2014-10-10
  • 出版日期:
文章二维码