口蹄疫病毒O/CHN/Mya98/33-P株前导蛋白对病毒感染性的影响

Foot-and-mouth disease virus O/CHN/Mya98/33-P strains leading protein effects on the virus infection

摘要:

【目的】研究口蹄疫病毒(Foot-and-mouth disease virus，FMDV)前导蛋白对于病毒感染性的影响。【方法】利用反向遗传操作技术，以O/CHN/93现用疫苗株的感染性克隆为骨架，分别构建了含口蹄疫病毒中国分离株O/CHN/Mya98/33-P和O/CHN/Mya98/HN1前导蛋白的两株嵌合全长cDNA感染性克隆，采用实时荧光定量PCR检测两株不同的嵌合病毒感染猪肾原代细胞后细胞内IFNβ和OAS mRNA的转录情况。【结果】嵌合病毒rOHN1Lab增殖能力较弱，其对应诱导产生的IFNβ和OAS mRNA含量较高，而嵌合毒rO33Lab相对于rOHN1Lab增殖能力较强，他们诱导产生的IFNβ和OAS mRNA含量较低。【结论】研究表明口蹄疫病毒中国分离株O/CHN/Mya98/33-P前导蛋白具有更强的抗IFNβ mRNA转录的能力，该研究能够为进一步鉴定FMDV前导蛋白抗宿主先天性免疫的关键性位点奠定基础。

Abstract:

[Objective] This study is to research the influence of foot-and-mouth disease virus (FMDV) leader protein for virus infectivity. [Methods] Two full-length infected cDNA clones were constructed based on O/HN/93 strain cDNA using reverse genetic technique which contain the leader protein of Chinese isolates O/CHN/Mya98/33-P and O/CHN/Mya98/HN1. Though real-time fluorescent quantitative PCR detecting the situation of IFNβ and OAS mRNA transcription, when pig kidney primary cells were infected the two different chimeric viruses. [Results] We found that the proliferation ability of chimeric virus rOHN1Lab is weaker, and the content of IFNβ and OAS it induced is higher. However, the proliferation ability of chimeric virus rO33Lab relative to rOHN1Lab is higher, and the content of IFNβ and OAS which induced is lower. [Conclusion] This study suggests that the leader protein of the Foot and mouth disease Chinese isolates O/CHN/Mya98/33-P have the stronger ability to resist IFNβ mRNA transcription, which lay the foundation for further identification of FMDV leader protein critical sites for resisting host innate immunity.