代谢工程改造大肠杆菌合成D-1,2,4-丁三醇

Biosynthesis of D-1,2,4-butanetriol by an engineered Escherichia coli

摘要:

【目的】D-1,2,4-丁三醇是一种四碳的多元醇，在军事和医药领域具有广泛的应用。为实现生物法一步转化生产D-1,2,4-丁三醇，对Escherichia coli W3100的木糖代谢途径进行改造。【方法】将来源于柄杆菌的D-木糖脱氢酶基因xylB和恶臭假单胞菌的苯甲酰甲酸脱羧酶基因mdlC克隆至E. coli W3100，得到重组菌E. coli (pEtac-mdlC-tac-xylB)。在此基础上对重组菌代谢木糖合成D-1,2,4-丁三醇的能力进行考察。【结果】在30 °C下，以30 g/L D-木糖为底物，重组菌E. coli (pEtac-mdlC-tac-xylB)的D-1,2,4-丁三醇产量达到了0.9 g/L，摩尔转化率为4%。【结论】实现了D-1,2,4-丁三醇的一步法发酵生产，为国内开展相关研究奠定了坚实的基础。

Abstract:

[Objective] D-1,2,4-butanetriol is widely used in military and medicine industry. To realize the biosynthesis of D-1,2,4-butanetriol from D-xylose directly, xylose metabolism of Escherichia coli W3100 was modified. [Methods] The D-xylose dehydrogenease gene xylB from Caulobacter sp. and benzolyformate decarboxylase gene mdlC from Pseudomonas putida were expressed in E. coli W3100, resulted in a recombinant E. coli (pEtac-mdlC-tac-xylB). [Results] When cultivated with 30 g/L D-xylose at 30 °C for 48 h, the titer and molar yield of D-1,2,4-butanetriol reached 0.9 g/L and 4%. [Conclusion] Our results may serve as a start-point for further genetic engineering to enhance the titer of D-1,2,4-butanetriol.