Abstract:This paper focuses on the teaching reform of design and development of the Microbiology Tutor System by the Microbiology course group of Zhejiang University of Technology. The Microbiology Tutor System has more than 3 800 questions, abundant kinds of questions and functions of online practice and exams. Meanwhile, the system emphasizes function of tutor help, gives targeted assistance to students during the network learning, integrates functions of assignments on network and online submit, which can reflect students learning and teachers teaching in many ways, multi-level, and acquires preferably effect of the network-assisted teaching in the microbiology course. Finally, some problems in network assistant teaching and how to solve these problems have been discussed.