CAS平板覆盖法检测氢氧化细菌铁载体

Detection of siderophore production from hydrogen-oxidizing bacteria with CAS overlay plate method

摘要:

【目的】用CAS平板覆盖法检测氢氧化细菌铁载体，解决通用CAS琼脂平板法中十六烷基三甲基溴化铵对真菌和某些细菌的生长抑制问题。【方法】将改良的CAS检测培养基覆盖在长满菌落的无铁培养基上，生长抑制问题因微生物未与十六烷基三甲基溴化铵直接接触而解决。【结果】3株氢氧化细菌SDW-5、SDW-9和AaP-13均能产生单菌落，加入CAS检测培养基1 h后，菌落周围产生明显的铁载体晕圈。【结论】本方法成功解决了生长抑制问题，可以作为检测微生物铁载体的通用方法。

Abstract:

[Objective] CAS(Chrome azurol S) overlay plate method was used for the detection of siderophore from hydrogen-oxidizing bacteria, to address the problem of growth inhibition for fungi and certain bacteria caused by hexadecyltrimethyl-ammonium bromide (HDTMA) in the popular CAS assay. [Methods] We just need to coverage the modified CAS medium on iron-free culture agar plates covered with colonies, then the problem of growth inhibition could be removed, due to the microorganisms tested with no direct contact with the hexadecyltrimethyl-ammonium bromide (HDTMA). [Results] Three strains of hydrogen-oxidizing bacteria, SDW-5, SDW-9, AaP-13 could form single colony, the siderophore halo around the colonies will form in an hour after a modified CAS medium is covered on iron-free culture agar plates. [Conclusion] This method successfully avoided growth inhibition problems, hence it could become a universal method for the detection of microorganisms siderophores.