细菌中黄素单核苷酸核糖开关研究进展

Research progress on Flavin mononucleotide riboswitch in bacteria

摘要:

核糖开关(Riboswitch)具有RNA结构，是位于mRNA 5′非编码区的RNA传感器。在无任何蛋白质因子参与下，可特异性地直接结合代谢产物，使自身构象发生相应变化，启动或阻断mRNA的转录、翻译、拼接等过程来调控基因的表达。某些Riboswitch可应用于抗菌药物研发。本文综述了Flavin mononucleotide riboswitch (FMN riboswitch)三维结构、基因表达调控的机制及热力学、动力学的研究进展，为基于FMN riboswitch的合理药物设计奠定了基础，并对开发新一代抗菌药物进行了展望。

Abstract:

Riboswitches are structured RNA domains usually residing in the 5′-untranslated region of messenger RNAs as RNA sensor elements, which can bind specifically and directly to certain metabolites to induce corresponding changes in their conformation and activate or inhibit the transcription, translation, and splicing of mRNA to regulate gene expression without the need for any regulatory factors. Some riboswitches can be used as novel targets for antibacterial drug discovery. This article reviews the structure of the Flavin mononucleotide (FMN) riboswitch, the mechanism of regulation of gene expression and thermodynamic, kinetic studies for the FMN riboswitch. The design and screen for the new generation of antibiotics based on FMN riboswitch are also discussed.