内蒙古鄂尔多斯一古盐湖真菌多样性及其功能酶的初步筛选

Identification of a parasitic Hirsutella Pat. of Bombyx mori Linnaeus. and sporulation conditions optimization

摘要:

【目的】探索内蒙古鄂尔多斯一古盐湖土壤沉积物和湖水样品中的真菌多样性，并初步筛选出产功能酶的活性菌株。【方法】采用含有2.5%、5%、10%及15% NaCl的3种分离培养基，利用稀释平板法分离可培养真菌，基于形态学和ITS序列分析对获得菌株进行系统分类学分析。利用6种筛选培养基定性检测盐湖真菌的产酶活性。【结果】共分离到2 121株真菌，共45个形态种，根据形态学研究和ITS序列的系统分类学分析将其归类为27个属，优势属为曲霉属(Aspergillus)。功能酶筛选结果表明，45个形态种中有22个形态种具有产酶活性，其中6个形态种只产蛋白酶，6个只产纤维素酶，2个只产复合酶，1个只产淀粉酶。有4个形态种可同时产3种酶，有3个可同时产2种酶。【结论】内蒙古鄂尔多斯地区盐湖中真菌多样性丰富，产酶特性良好，研究工作为耐(嗜)盐真菌资源的进一步开发和利用提供依据。

Abstract:

[Objective] The diversity of fungi in soils and sediments of Erdos paleo salt lake in Inner Mongolia and the strains containing functional enzymes were identified. [Methods] Three isolation media containing designed concentrations of NaCl were used to separate and cultivate fungi. Based on analysis of morphology and ITS sequences, the phylogenetic of tested bacterial strains was performed. The enzyme activity in the salt lake fungi was qualitatively determined using 6 kinds of screened media. [Results] Total 2 121 strains of fungi were separated from the salt lake. These strains were divided into 45 morphospecies and 27 generas based on the analysis of morphology and ITS sequences. The dominant genus was Aspergillus. Twenty-two of 45 morphospecies produced enzyme, 6 produced protease(s), 6 produced cellulase(s), 2 produced compound enzyme, 1 produced amylase enzymes and 1 produced lipase. Meanwhile, 4 morphospecies could simultaneously produce three. [Conclusion] The fungi from the Erdos salt lake in Inner Mongolia are rich in diversity and can produce various enzymes. This study provides a basis for the further characterization and utilization of halophilic microbes.