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阿特拉津降解菌Acinetobacter sp. DNS32对无机氮源的响应
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黑龙江省高校长江学者后备支持计划项目(No. 2012CJHB001, CSCP);黑龙江省高校创新团队建设计划项目(No. 2013TD003)


Response of an atrazine-degrading bacterium strain Acinetobacter sp. DNS32 to inorganic nitrogen source
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    【目的】研究Acinetobacter sp. DNS32的生长、阿特拉津降解能力和降解基因转录水平的表达对无机氮素的响应关系,为菌株的工程应用提供指导与理论基础。【方法】以Acinetobacter sp. DNS32为对象,采用摇瓶法研究菌株在阿特拉津培养基中菌株生长情况及降解能力对外加硝态氮与铵态氮的响应关系,利用荧光定量PCR技术检测DNS32降解基因表达量对外加无机氮源的响应关系。【结果】外加无机氮源可以促进DNS32菌株的生长,提高阿特拉津降解能力,无机氮源对DNS32菌株的trzN、atzB和atzC 3种降解基因表达均有促进作用,加入无机氮源的试验处理中DNS32菌株trzN基因的表达量最高可达对照的11.252±2.408倍,推断DNS32菌株的这3种降解基因所编码的酶是稳定表达的组成酶。【结论】DNS32降解阿特拉津不受“氮饥饿”诱导机制调控,且无机氮源的存在对菌株的生长与降解有促进作用,因此菌株在土壤修复实践中具有广阔的应用前景。

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    [Objective] The diversity of fungi in soils and sediments of Erdos paleo salt lake in Inner Mongolia and the strains containing functional enzymes were identified. [Methods] Three isolation media containing designed concentrations of NaCl were used to separate and cultivate fungi. Based on analysis of morphology and ITS sequences, the phylogenetic of tested bacterial strains was performed. The enzyme activity in the salt lake fungi was qualitatively determined using 6 kinds of screened media. [Results] Total 2 121 strains of fungi were separated from the salt lake. These strains were divided into 45 morphospecies and 27 generas based on the analysis of morphology and ITS sequences. The dominant genus was Aspergillus. Twenty-two of 45 morphospecies produced enzyme, six produced protease(s), six produced cellulase(s), two produced compound enzyme, one produced amylase enzymes and one produced lipase. Meanwhile, four morphospecies could simultaneously produce three. [Conclusion] The fungi from the Erdos salt lake in Inner Mongolia are rich in diversity and can produce various enzymes. This study provides a basis for the further characterization and utilization of halophilic microbes.

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王志刚,张颖,郭火生,伊欢. 阿特拉津降解菌Acinetobacter sp. DNS32对无机氮源的响应[J]. 微生物学通报, 2014, 41(8): 1541-1546

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  • 在线发布日期: 2014-08-07
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