大肠杆菌O157显色培养基检测效果的评价

Evaluation of the detecting effect for chromogenic Escherichia coli O157 medium

摘要:

【目的】评价显色培养基对大肠杆菌O157:H7 (Escherichia coli O157:H7)的检测效果。【方法】本实验室研制的大肠杆菌O157显色培养基(HKM)，与国外梅理埃、科玛嘉及国内厂家的同类产品及传统培养基CT-SMAC作比较，对相关菌株以及污染样品和实际样品进行对比测试。【结果】实验室研制的HKM大肠杆菌O157显色培养基与科玛嘉同类产品在特异性、灵敏度及检测效果方面均无明显差异，均优于梅里埃、国内厂家产品及CT-SMAC。【结论】HKM大肠杆菌O157显色培养基具有高检出率及高特异性的特点，具有较好的应用价值和前景。

Abstract:

[Objective] To evaluate the efficacy of chromogenic medium for Escherichia coli O157:H7. [Methods] A new Escherichia coli O157 chromogenic medium (HKM) was compared with CHROMagar, BioMerieux, Domestic company and CT-SMAC for detection of Escherichia coli O157 by reference strains, artificially contaminated samples and natural samples. [Results] The selectivity, highly specificity and detection efficiency of HKM were as same as that of CHROMagar, and were better than that of BioMerieux, Domestic company and CT-SMAC. [Conclusion] The HKM E. coli O157 chromogenic medium has a good effect and invaluable in detecting E. coli O157:H7, because it has high specificity and selectivity.