抗肿瘤银杏内生真菌Aspergillus oryzae YX-5发酵 培养基的优化

Optimization of the fermentation medium of an antitumor endophytic fungus Aspergillus oryzae YX-5 isolated from Ginkgo biloba

摘要:

【目的】对一株具有抗肿瘤活性的银杏内生真菌Aspergillus oryzae YX-5的发酵培养基进行优化。【方法】以发酵后的菌体干重、粗提物质量和粗提物抗肿瘤活性为指标，通过单因素实验选出合适的碳源和氮源，再以选出的碳源、氮源以及K2HPO4、MgSO4·7H2O、KCl共 5个因素进行正交实验，确定出最适宜的发酵培养基配方。【结果】YX-5的最佳发酵培养基配方为葡萄糖45 g/L，蛋白胨8 g/L，K2HPO4 0.5 g/L，MgSO4·7H2O 0.2 g/L，KCl 1 g/L，FeSO4 0.01 g/L。优化后菌体干重、代谢物的产量和抗肿瘤活性分别提高了41.88%、226.52%和19.31%。【结论】通过对米曲霉菌(Aspergillus oryzae) YX-5的发酵培养基进行优化，明显提高了粗提物产量和抗肿瘤活性，这对后期规模化发酵中减小发酵规模和降低工作量十分有利。

Abstract:

[Objective] To optimize the fermentation medium of an antitumor endophytic fungus isolated from Ginkgo biloba. [Methods] In this study, the dry weight of mycelia, weight and antitumor activity of crude extract were used as standards to screen out the best carbon source and nitrogen source through single factor experiment. Then the culture medium recipe was optimized by the orthogonal test in five factors and four levels. [Results] The best culture medium recipe was: glucose 45 g/L, peptone 8 g/L, K2HPO4 0.5 g/L, MgSO4·7H2O 0.2 g/L, KCl 1 g/L, FeSO4 0.01 g/L. After optimization, the dry weight of mycelia, weight and antitumor activity of crude extract were increased by 41.88%, 226.52% and 19.31% respectively. [Conclusion] The production and antitumor activity of crude extract was significantly increased after optimization, which might be beneficial to reduce the fermentation scale and workload in scale up fermentation later.