微生物完整基因组测定中的Gap closure策略

Strategies of gap closure in complete microbial genome sequencing

摘要:

微生物基因组空缺区域(Gap)中可能存在重要的生物学信息，如果无法补齐所有Gap，不仅不能获得完整的基因组图谱，还会给后续的基因组信息解读造成很大困难。而基因组空缺区域填充(Gap closure)是获得微生物基因组完成图的关键，本文结合作者以及借鉴上海人类基因组研究中心在微生物基因组Gap closure中的经验，针对微生物基因组Gap closure常用的6种策略：参考序列比对、多引物PCR、基因组步移、基因组文库克隆末端测序、末端配对(Paired-End)以及基因组光学图谱技术进行综述。

Abstract:

Gaps in microbial genome sequences may lead to loss of important biological information and cause trouble for further interpretation of genetic information. Gap closure is thus a critical step in completely genome finishing of microorganisms. In this review, we critically summarize six major strategies for gap filling of microbial genomes, including reference alignment, multiplex PCR, genome walking, genome library clone-end sequence, paired-end and whole genome mapping.