科微学术

微生物学通报

一株泰乐菌素高效降解菌的分离鉴定及其降解特性
CSTR:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

国家自然科学基金项目(No. 21067009)


Isolation and identification of a tylosin-degrading strain and its degradation characteristics
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    【目的】从长期堆放泰乐菌素药渣附近的土壤中分离出泰乐菌素降解菌,并考察其对泰乐菌素的降解特性。【方法】采用梯度驯化、划线分离法筛选出泰乐菌素优势降解菌,通过形态观察、生理生化特征和16S rRNA基因序列分析方法对其进行系统发育分析及菌种鉴定,并考察菌株对泰乐菌素的降解特性。【结果】从长期堆放泰乐菌素药渣的土壤中分离得到1株泰乐菌素高效降解菌,命名为TS1,其为革兰氏阴性杆菌,菌落形态呈圆形,乳白色,表面光滑,不透明,边缘整齐,鉴定为越南伯克霍尔德氏菌(Burkholderia vietnamiensis)。该菌株在温度35 °C、pH 7.0的条件下培养72 h,对初始浓度为300 mg/L泰乐菌素的降解率可达99% 以上。【结论】说明菌株TS1对泰乐菌素具有良好的降解特性,可用于生物修复被泰乐菌素废渣废水污染的生态环境。

    Abstract:

    [Objective] This study was to isolate a tylosin-degrading strain from the soil deposited by tylosin pharmaceutical waste and investigated its degradation characteristics. [Methods] The strain was identified based on the morphology observation, experimental results of physiology and biochemsitry, and then the identification and phylogenetic analysis of the isolated strain was performed by 16S rRNA gene sequence, also, the characteristics of the strain in degradation of tylosin was investigated. [Results] A strian named TS1 was newly isolated from the soil deposited by tylosin pharmaceutical waste. The cells of the isolate were Gram-negative rods and the colony morphology was round, oyster white, opaque, and smooth with regular edge. The isolate was identified as Burkholderia vietnamiensis. This strain was capable of degrading 99% of tylosin in medium with an initial concentration of 300 mg/L after 72 h of incubation under conditions of the initial pH 7.0 and 35 °C. [Conclusion] The isolated strain has a high ability to degrade tylosin, and may be used for bioremediation of environment contaminated by solid or liquid waste containing tylosin residue.

    参考文献
    相似文献
    引证文献
引用本文

孙瑞珠,马玉龙,张娟,孙永刚,王艳,王敏,牛海亚. 一株泰乐菌素高效降解菌的分离鉴定及其降解特性[J]. 微生物学通报, 2014, 41(4): 681-690

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2014-04-04
  • 出版日期:
文章二维码