两株生防芽孢细菌筛选、鉴定及拮抗研究

Screening and identification and antagonism research of two biocontrol bacillus strains

摘要:

【目的】筛选出广谱、高效的生防芽孢细菌，并对其拮抗作用进行研究。【方法】以8种植物病原真菌为靶标菌，通过皿内拮抗和发酵液拮抗能力的测定筛选出2株广谱性和高效性的芽孢细菌B06和B07。【结果】B06对8种植物病原真菌的R2/R1为0.4?1.8，无菌滤液对8种植物病原真菌的抑制率为66.7%?87.5%。B07对8种植物病原真菌的R2/R1为0.23?1.21，无菌滤液对8种植物病原真菌的抑制率为55.56%?81.25%。经16S rRNA序列鉴定，菌株B06和B07都被鉴定为解淀粉芽孢杆菌。【结论】芽孢细菌能够抑制多种植物病原真菌，具有较好的抑病作用。广谱和高效芽孢细菌的筛选在农业生物防治方面具有很大的开发和应用价值。

Abstract:

[Objective] This study aimed to screen biocontrol bacillus strains with broad-spectrum and highly efficient antagonistic activity against plant pathogen fungi, and further to study the antagonist function. [Methods] Present study used eight kinds of plant pathogenic fungi as target bacteria to screen superior bacillus strains from 9 candidate strains, the method of flat confrontation, antagonistic activity determination of fermentation filtrate were used. [Results] Two bacillus strains B06 and B07 were found with the characteristics of broad-spectrum and highly efficient antagonistic activity. B06 was more effective in inhibiting 8 pathogenic fungi than other strains, with R2/R1 ranging from 0.4 to 1.8, and the inhibition rate of sterile fermentation filtrate from 66.7% to 87.5%. B07 took second place in inhibiting 8 pathogenic fungi than other strains, with R2/R1 ranging from 0.23 to 1.21, and the inhibition rate of sterile fermentation filtrate from 55.56% to 81.25%. Bacillus strains B06 and B07 were both identified as Bacillus amyloliqueaciens with the methods of 16S rDNA sequence analysis. [Conclusion] The result indicates that bacillus strain had the ability to inhibit kinds of plant pathogen fungi. Screening of bacillus strains with broad-spectrum and highly efficient antagonistic activity has a great development and application value in agricultural biological control.