Lysobacter antibioticus 13-1菌株抗菌物质鉴定及对水稻白叶枯病的防治效果

Control of rice bacterial leaf blight by antibacterial substances from Lysobacter antibioticus strain 13-1

摘要:

【目的】为了明确对水稻白叶枯病具有优质防效的生防细菌——抗生素溶杆菌13-1菌株抗菌物质及对白叶枯病的防治效果。【方法】研究采用高效液相色谱、质谱、13C核磁共振谱、氢核磁共振谱、电喷雾质谱等分析方法。【结果】13-1产生4种抑菌活性组分：6-甲氧基-10-氧基-1-吩嗪醇、吩嗪、吩嗪-1-羧酸及1-羟基-6-甲氧基吩嗪。4种吩嗪类物质对水稻白叶枯病原细菌均具有抑菌活性。田间小区试验表明，菌株13-1发酵液对水稻白叶枯病的防效在60%以上。【结论】研究明确了菌株13-1产生的抗菌物质为吩嗪类物质，这是国内关于吩嗪类物质控制水稻白叶枯病的首次报道。

关键词:根际细菌13-1，抗细菌物质，水稻白叶枯病，生物防治

Abstract:

[Objective] To clarify the antibacterial activity of Lysobacter antibioticus strain 13-1 against rice bacterial blight. [Methods] Four antibiotic compounds were isolated from the fermentation extracts of the strain 13-1 by sephadex LH-20 column chromatography, reverse C-18 Silica gel and high performance liquid chromatography (HPLC). [Results] These purified compounds were identified as 6-Methoxy-1-phenazinol-10-oxide, Phenazine, 1-Phenazinecarboxylic acid and 1-Hydroxy-6-methoxyphenazine by nuclear magnetic resonance (NMR), ESI-Mass spectrum analysis. Four phenazine analogs strongly inhibited mμLtiple Xoo strains growth. The protective efficacy of strain 13-1 was above 60% in field plot compared to controls. [Conclusion] The antimicrobial substances of strain 13-1 was phenazine substances that have the inhibition potential against rice bacterial blight.

Key words:Rhizosphere strain 13-1, Antibacterial compounds, Rice bacterial blight, Biocontrol