产漆酶菌株筛选及一株产酶菌株的优化与鉴定

Screening of fungal strains with laccase and optimization and identification of the strain with high-yield laccase

摘要:

【目的】从26株真菌菌株中筛选高产漆酶菌株。【方法】采用愈创木酚法进行产漆酶菌株的筛选，通过正交实验对筛选出的高产菌株进行优化，并通过形态学和分子系统学对菌株进行鉴定。【结果】26株真菌菌株中有4株可产生漆酶，其中菌株H52.1为产漆酶最好菌株；菌株H52.1产漆酶优化培养基碳源为可溶性淀粉，氮源为硝酸铵，pH为8，金属离子为Ca2+；经鉴定，该菌株为大孢戴氏霉。【结论】大孢戴氏霉在产漆酶方面值得进一步研究开发。

Abstract:

[Objective] To obtain the fungal stain with high-yield laccase. [Methods] Strains producing laccase were screened out by the guaiacol method. Then the strain with the high-yield laccase screened was optimized by the orthogonal experiment design and identified using the morphological characters and the molecular systematics. [Results] Four strains could produce laccase in these 26 fungal strains, and the strain H52.1 was the best high-yield strain, and its optimal medium components and conditions were as follows, the optimal carbon source was soluble starch, nitrogen source was ammonium nitrate, pH value was 8 and metal ion was Ca2+. The result of identification showed that the strain H52.1 was Taifanglania major. [Conclusion] Taifanglania major has been worth doing further research and exploitation in the laccase development.