S-腺苷甲硫氨酸合成酶对埃博霉素生物合成的影响

Influence of S-adenosylmethionine synthetase on epothilone biosynthesis

摘要:

【目的】研究S-腺苷甲硫氨酸合成酶(SAMs)对埃博霉素生物合成的影响。【方法】通过向发酵培养基中添加抑制剂和促进剂，比较分析纤维堆囊菌中SAMs的活性变化以及埃博霉素的产量变化。【结果】在埃博霉素的合成期，SAMs的活性较高。加入抑制剂吲哚乙酸(IAA)之后，SAMs的活性和埃博霉素的产量都不同程度的降低，而加入促进剂对甲苯磺酸钠(p-TSA-Na)之后，SAMs的活性和埃博霉素的产量在不同程度上都有提高。在纤维堆囊菌的次级代谢中，SAMs活性与埃博霉素的生物合成量呈正相关。【结论】S-腺苷甲硫氨酸合成酶在纤维堆囊菌的埃博霉素生物合成过程中发挥了重要的作用。

Abstract:

[Objective] To study the relationship between S-adenosylmethionine synthetase (SAMs) activity and the biosynthesis of epothilone. [Methods] We analyzed the relationship between the activities of SAMs and the yields of epothilone by the addition of inhibitor and accelerator. [Results] SAMs showed high activities in the period of the biosynthesis of epothilone. SAMs activities and the yields of epothilones both reduced with the indole-3-acetic acid addition and increased with the sodium p-toluenesulfonate addition. The SAMs activities were positive correlated with the yields of epothilone when the inhibitor or accelerator was added. [Conclusion] SAMs play an important role in the biosynthesis of epothilone in Sorangium cellulosums.