Sphingobacterium bambusaue及其紫外诱变菌株的石油降解功能

Petroleum degradation capacity of Sphingobacterium bambusaue and its UV-mutants

摘要:

【目的】研究Sphingobacterium bambusaue及其紫外诱变菌株的石油降解功能。【方法】紫外诱变后筛选石油降解高效菌株; 以不同石油浓度、pH值及盐浓度优化培养条件, 用重量法检测高效菌株石油降解率。【结果】发现菌株S. bambusaue在石油降解培养基中培养5 d的石油降解率为25.86%, UV诱变高效菌株IBFC2009-S3培养5 d的石油降解为42.85%, 比始发菌株提高65.7%; UV诱变菌株IBFC2009-S3的优化培养条件为石油浓度0.5 g/L、pH值7.0以及NaCl质量浓度为10 g/L, 其石油降解率可达50.51%。【结论】首次报道S. bambusaue具有石油降解功能; 紫外诱变获得的菌株S3的石油降解能力较强。

Abstract:

[Objective] Crude oil degradation by Sphingobacterium bambusaue and its UV mutants were studied. [Methods] The efficient crude oil degrading strains were obtained by UV mutagenesis, and the crude oil degradation rate was tested by gravimetric method in the culture conditions of different crude oil concentration, pH and salt concentration. [Results] The crude oil degradation rate of the strain Sphingobacterium bambusaue and UV mutant IBFC2009-S3 cultured in crude oil medium for 5 d was 25.86% and 42.85% respectively, and the crude oil degradation rate of UV mutant IBFC2009-S3 increased 65.7% than that of the originating strains; the crude oil degradation rate of the strain IBFC2009-S3 was 50.51% in the optimum condition which was 0.5 g/L crude oil, pH 7.0, and 10 g/L NaCl. [Conclusion] Crude oil degradation by Sphingobacterium bambusaue and its UV mutants IBFC2009-S3 have the higher ability of crude oil degradation.