蒺藜内生真菌发酵液抗菌及抗肿瘤活性初步研究

Antagonism on microorganisms and cancer cells by the fermentation broth of endophytic fungi from Tribulus terrestris L.

摘要:

【目的】筛选出具有抗菌和抗肿瘤活性的蒺藜内生真菌。【方法】采用牛津杯法、稻瘟菌模型及肿瘤细胞模型评价蒺藜内生真菌PDB和察氏培养基发酵产物的抗菌活性和肿瘤细胞毒性。【结果】PDB培养基发酵液和察氏培养基发酵液抑菌圈直径大于10 mm的菌株分别占总菌株数的19.05%和23.81%。PDB培养基发酵液和察氏培养基发酵液对稻瘟菌的最小抑制浓度(MIC)低于10%的菌株分别占总菌株数的19.05%和47.61%。对肿瘤细胞抑制率高于50%的PDB发酵产物占PDB发酵产物总数的52.38%, 而对肿瘤细胞抑制率高于50%的察氏发酵产物占察氏发酵产物总数的28.57%。【结论】部分蒺藜内生真菌的发酵产物具有抗菌和抗肿瘤活性。

Abstract:

[Objective] The aim of the present study is to investigate the anti-microorganisms and anticancer activities of endophytic fungi isolated from Tribulus terrestris L.. [Methods] Endophytic fungi were cultured in PDB or Czapek medium to obtain different liquid fermentation. The activities of fermentative products were tested by using Oxford plate assay system, Magnaporthe oryzae P-2b model and cancer cell model. [Results] The strains with their inhibition zone diameter of the PDB and Czapek broth greater than 10 mm were accounted for 19.05% and 23.81% respectively of the total strains. The strains of which the PDB broth and the Czapek broth with the MIC (Minimum inhibitory concentration) of Magnaporthe oryzae lower than 10%, were accounted for 19.05% and 47.61% respectively of the total strains. The PDB fermentation broth with the inhibitory action on cancer cells over 50% was accounted for 52.38% of the total PDB broth, and the Czapek fermentation broth with the inhibitory action on cancer cells over 50% was accounted for 28.57% of the total Czapek broth. [Conclusion] A certain amount of fermentative broth of endophytic fungi isolated from Tribulus terrestris L. had effective anti-microorganisms and anticancer activities.