梭柄松苞菇多糖组分的部分理化性质及对S-180瘤抑制活性研究

Parts of physical and chemical properties of the polysaccharides from Catathelasma ventricosum (Pk.) Sing. Armillaria ventricosa Peck and their inhibitory effect on S-180 tumor

摘要:

【目的】分离和纯化梭柄松苞菇子实体多糖, 并对其结构和抗肿瘤活性进行研究。【方法】采用热水浸提法提取梭柄松苞菇子实体多糖, 采用DE-52纤维素柱和Sephadex G-100进行多糖的分离纯化, HPGPC测定多糖分子量, HPLC测定单糖组成, 红外光谱进行结构分析, 甲基化实验测定连接方式, 核磁共振测定异头碳构型, 采用S-180荷瘤小鼠模型对梭柄松苞菇子实体多糖抗肿瘤活性进行研究, 并测定了荷瘤小鼠血清细胞因子TNF-α、IL-2和IL-6的含量。【结果】从梭柄松苞菇子实体中分离纯化得到多糖命名为CVP-A, 结果表明, CVP-A平均分子量为10 289, 单糖组成主要为葡萄糖, 还含有少量木糖, 含量比约为15:2, 甲基化实验, 核磁共振以及红外光谱分析结果表明CVP-A主链为(1→4)-α-D-葡聚糖, 侧链连接在主链葡萄糖残基2号碳位置。S-180荷瘤小鼠模型实验表明, CVP-A对实验性S-180实体瘤具有较好的抑制作用, 连续10 d腹腔注射25、50和75 mg/(kg·d) CVP-A的昆明小鼠的肿瘤抑制率分别为31.99%、42.68%和60.18%。小鼠血清细胞因子测定表明, CVP-A能提高荷瘤小鼠血清TNF-α、IL-2和IL-6的含量。【结论】梭柄松苞菇多糖可能作为一个潜在的抗癌药物而开发利用。

Abstract:

[Objective] This article mainly studied the extraction, purification, structure and anti-tumor effects of a novel polysaccharide purified from the fruit bodies of Catathelasma ventricosum (Pk.) Sing. Armillaria ventricosa Peck. [Methods] The polysaccharides of Catathelasma ventricosum (Pk.) Sing. Armillaria ventricosa Peck were extracted with boiling water and purified by DE-52 column and Sephadex G-100 column. The molecular weight, monosaccharide composition and backbone structure were analysed by HPGPC, HPLC, FT-IR, methylation and NMR; the anti-tumor effect was investigated by S-180 tumor-bearing mice and evaluated by the cytokine levels of IL-2, IL-6 and TNF-α in serum samples of the tumor-bearing mice. [Results] A novel polysaccharide was extracted from the fruit bodies of Catathelasma ventricosum (Pk.) Sing. Armillaria ventricosa Peck and named CVP-A, the results had showed that the molecular weight of CVP-A was 10 289, the monosaccharide composition of CVP-A were mainly composed of glucose and a small amount of xylose, with a ratio near 15:2. The results of HPGPC, HPLC, FT-IR, methylation and NMR revealed that CVP-A had a backbone structure of (1→4)-α-D-glucopyranose which branched at the O-2 of the glucose residues. The results of S-180 tumor-bearing mice model had showed that CVP-A had a good inhibiting effect on the experimental S-180 solid tumor, after injection of 25 mg/(kg?d), 50 mg/(kg?d), 75 mg/(kg?d) CVP-A for ten days, the three Kunming mice group had a tumor inhibition ratio of 31.99%, 42.68%, 60.18%, in which the cytokines levels of IL-2, IL-6 and TNF-α in S-180 tumor-bearing mice were increased significantly. [Conclusion] Catathelasma ventricosum (Pk.) Sing. Armillaria ventricosa Peck may be a potential source for anti-tumor development.